Document 0065
 DOCN  M9490065
 TI    Anti-HTLV-I IgG in urine detected by sensitive enzyme immunoassay
       (immune complex transfer enzyme immunoassay) using a synthetic peptide,
       Cys-env gp46(188-224), as antigen.
 DT    9411
 AU    Hashida S; Hirota K; Kohno T; Ishikawa E; Department of Biochemistry,
       Medical College of Miyazaki, Japan.
 SO    J Clin Lab Anal. 1994;8(3):149-56. Unique Identifier : AIDSLINE
       MED/94322150
 AB    Antibody IgG to human T-cell leukemia virus type I (HTLV-I) in urine was
       detected by a sensitive enzyme immunoassay (immune complex transfer
       enzyme immunoassay) using a synthetic peptide, Cys-env gp46(188-224), as
       antigen, the sensitivity and specificity of which were 100 and 98.5%,
       respectively, using serum samples. Anti-HTLV-I IgG in urine was reacted
       simultaneously with 2,4-dinitrophenyl-bovine serum albumin-Cys-env
       gp46(188-224) conjugate and Cys-env gp46(188-224)-beta-D-galactosidase
       (Escherichia coli) conjugate. The complex formed, consisting of the
       three components, was trapped onto polystyrene balls coated with
       affinity-purified (anti-2,4-dinitrophenyl group) IgG, eluted with
       epsilon N-2,4-dinitrophenyl-L-lysine and transferred to polystyrene
       balls coated with affinity-purified (anti-human IgG gamma-chain) IgG.
       Finally, bound beta-D-galactosidase activity was assayed by fluorometry.
       Thirty-one urine samples from seropositive subjects and 100 urine
       samples from seronegative subjects were tested. The sensitivity and
       specificity were 87 and 100%, respectively, with unconcentrated urine
       samples and 94 and 100%, respectively, with approximately 10-fold
       concentrated urine samples. These results were superior to those by the
       conventional ELISA and gelatin particle agglutination test.
 DE    Adult  Aged  Amino Acid Sequence  Animal  Antigen-Antibody
       Complex/METABOLISM  Female  Human  HTLV-I Antibodies/*URINE  HTLV-I
       Antigens/*IMMUNOLOGY  IgG/*URINE  Immunoenzyme Techniques  Male  Middle
       Age  Molecular Sequence Data  Peptide Fragments/*IMMUNOLOGY  Rabbits
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).